Expression cloning of human B cell immunoglobulins.

@article{Wardemann2013ExpressionCO,
  title={Expression cloning of human B cell immunoglobulins.},
  author={Hedda Wardemann and Juliane Kofer},
  journal={Methods in molecular biology},
  year={2013},
  volume={971},
  pages={93-111}
}
The majority of lymphomas originate from B cells at the germinal center stage or beyond. Preferential selection of B cell clones by a limited set of antigens has been suggested to drive lymphoma development. However, little is known about the specificity of the antibodies expressed by lymphoma cells, and the role of antibody-specificity in lymphomagenesis remains elusive. Here, we describe a strategy to characterize the antibody reactivity of human B cells. The approach allows the unbiased… CONTINUE READING

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