Expression cloning of cDNAs that render cancer cells resistant to Pseudomonas and diphtheria toxin and immunotoxins.

@article{Brinkmann1995ExpressionCO,
  title={Expression cloning of cDNAs that render cancer cells resistant to Pseudomonas and diphtheria toxin and immunotoxins.},
  author={Ulrich Brinkmann and Elisabeth Brinkmann and Ira Pastan},
  journal={Molecular medicine},
  year={1995},
  volume={1 2},
  pages={
          206-16
        }
}
BACKGROUND Several immunotoxins in which antibodies are coupled to plant or bacterial toxins are now in clinical trials for the treatment of cancer. One of these is B3-LysPE38 in which MAb B3 which reacts with many human cancers, is coupled with a genetically modified form of Pseudomonas exotoxin (PE). MATERIALS AND METHODS To investigate how cells can become resistant to PE-derived immunotoxins, we constructed an immunotoxin-sensitive MCF-7 breast cancer cell line that contains SV40 T… CONTINUE READING