Expression cloning of a human dual-specificity phosphatase.

  title={Expression cloning of a human dual-specificity phosphatase.},
  author={Teruo Ishibashi and Donald P Bottaro and Andrew M-L Chan and Tohru Miki and Stuart A. Aaronson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={89 24},
Using an expression cloning strategy, we isolated a cDNA encoding a human protein-tyrosine-phosphatase. Bacteria expressing the kinase domain of the keratinocyte growth factor receptor (bek/fibroblast growth factor receptor 2) were infected with a fibroblast cDNA library in a phagemid prokaryotic expression vector and screened with a monoclonal anti… CONTINUE READING