Expression cloning of SR-BI, a CD36-related class B scavenger receptor.

  title={Expression cloning of SR-BI, a CD36-related class B scavenger receptor.},
  author={Susan L. Acton and Philipp E. Scherer and Harvey F. Lodish and Monty Krieger},
  journal={The Journal of biological chemistry},
  volume={269 33},

SR-BII, an Isoform of the Scavenger Receptor BI Containing an Alternate Cytoplasmic Tail, Mediates Lipid Transfer between High Density Lipoprotein and Cells*

These studies show that SR-BII, an HDL receptor isoform containing a distinctly different cytoplasmic tail, mediates selective lipid transfer between HDL and cells, but with a lower efficiency than the previously characterized variant.

Scavenger receptor class B type I as a receptor for oxidized low density lipoprotein.

A potential function of SR-BI is demonstrated, in addition to its role in selective uptake of lipids, to mediate internalization of OxLDL by macrophages and suggest a central role for oxidized phospholipids in this process.

The Class B Scavenger Receptors SR-BI and CD36 Are Receptors for Anionic Phospholipids (*)

Using both direct binding and ligand competition assays in transfected cells, it is found that two class B scavenger receptors, SR-BI and CD36, can tightly bind PS and phosphatidylinositol (PI)-containing liposomes.

Expression Cloning of a Novel Scavenger Receptor from Human Endothelial Cells*

This study isolated, by expression cloning, the cDNA encoding a novel type of scavenger receptor expressed by endothelial cells (SREC), which mediates the binding and degradation of Ac-LDL.

Characterization of CLA-1, a Human Homologue of Rodent Scavenger Receptor BI, as a Receptor for High Density Lipoprotein and Apoptotic Thymocytes*

The results demonstrate that CLA-1, a close structural homologue of SR-BI, is also functionally related toSR-BI and may play an important role as a “docking receptor” for HDL in connection with selective uptake of cholesterol esters and an additional role in recognition of damaged cells is suggested.

Cd36, a class B scavenger receptor, functions as a monomer to bind acetylated and oxidized low‐density lipoproteins

The data suggest that this class B scavenger receptor may gain functionality for ligand binding, and/or ligand internalization, by formation of protein complexes at the cell surface, and the in vitro ligand‐binding assay represents a promising screen for identification of bioactive molecules targeting atherogenesis at the level of ligandbinding.

The Human Scavenger Receptor CD36

Flow cytometric analysis of the different glycosylation mutants expressed in mammalian cells established that gly cosylation is necessary for trafficking to the plasma membrane and showed that neither the nature nor the pattern of glycosolation is relevant to binding of modified low density lipoprotein.

Expression of human scavenger receptor class B type I in cultured human monocyte-derived macrophages and atherosclerotic lesions.

This study clearly demonstrates that hSR-BI is expressed in the lipid-laden macrophages in human atherosclerotic lesions, suggesting that it is very important to know its function and regulation in hMphi to understand the biological utility of this molecule.



Differentiation of binding sites on reconstituted hepatic scavenger receptors using oxidized low-density lipoprotein.

There is evidence that the reconstituted receptors either have several binding sites for each of the various ligands or are functionally different, despite the fact that they do not differ in their apparent molecular masses.

Coiled-coil fibrous domains mediate ligand binding by macrophage scavenger receptor type II

The type II scavenger receptor mediates endocytosis of chemically modified low-density lipoprotein with high affinity and specificity, similar to that of the type I receptor, and one or both of the extracellular fibrous domains are responsible for the unusual ligand-binding specificity of the receptor.

Two distinct receptors account for recognition of maleyl-albumin in human monocytes during differentiation in vitro.

It is reported that human monocytes express a second cellular surface receptor for maleyl-albumin that is distinct from the scavenger receptor, a useful adjunct to studies of cellular events mediated by the scavengers.

Isolation of the thrombospondin membrane receptor.

An 88-kD membrane glycoprotein present in platelets, endothelial cells, monocytes, and a variety of human tumor cell lines that is the membrane binding site for TSP is identified and isolated and may function as the cellular TSP receptor.

Scavenger function of sinusoidal liver cells. Acetylated low-density lipoprotein is endocytosed via a route distinct from formaldehyde-treated serum albumin.

The results indicate that the scavenger receptors for these two ligands are distinct from each other but similarly sensitive to polyanionic compounds.