Expression cloning of GABAB receptors uncovers similarity to metabotropic glutamate receptors

@article{Kaupmann1997ExpressionCO,
  title={Expression cloning of GABAB receptors uncovers similarity to metabotropic glutamate receptors},
  author={Klemens Kaupmann and K Huggel and Jakob Heid and Peter Josef Flor and Serge François Bischoff and Stuart J Dr Mickel and Gary Mcmaster and Christof Angst and Helmut Bittiger and Wolfgang Froestl and Bernhard Bettler},
  journal={Nature},
  year={1997},
  volume={386},
  pages={239-246}
}
GABA (γ-amino-butyric acid), the principal inhibitory neurotransmitter in the brain, signals through ionotropic (GABAA/GABAC) and metabotropic (GABAB) receptor systems. Here we report the cloning of GABAB receptors. Photoaffinity labelling experiments suggest that the cloned receptors correspond to two highly conserved GABAB receptor forms present in the vertebrate nervous system. The cloned receptors negatively couple to adenylyl cyclase and show sequence similarity to the metabotropic… 

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The focus will be on nonclassical aspects of GABAB receptor phosphorylation, desensitization, downregulation, signaling, and association with a variety of protein partners.

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Heterodimerization is required for the formation of a functional GABAB receptor

TLDR
The cloning of a complementary DNA encoding a new subtype of the GABAB receptor (GABABR2), which is identified by mining expressed-sequence-tag databases, indicates that, in vivo, functional brain GABAB receptors may be heterodimers composed of GABABR1 and GABABR2.
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