Expression and spectroscopic characterization of the hydrogenosomal [2Fe-2S] ferredoxin from the protozoan Trichomonas vaginalis.

@article{Vidakovic1996ExpressionAS,
  title={Expression and spectroscopic characterization of the hydrogenosomal [2Fe-2S] ferredoxin from the protozoan Trichomonas vaginalis.},
  author={Momcilo Vidakovic and Grazyna Fraczkiewicz and Juris P. Germanas},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 25},
  pages={14734-9}
}
The heterologous expression and spectroscopic characterization of the [2Fe-2S] ferredoxin from the sexually transmitted human parasite Trichomonas vaginalis is described. Using oligonucleotide primers based on the deduced DNA sequence, the gene encoding the ferredoxin was amplified by polymerase chain reaction and cloned into a T7 RNA polymerase expression vector. Expression of the gene in Escherichia coli host HMS174(DE3) resulted in the high level production of the protein with the correctly… CONTINUE READING

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