Expression and site-directed mutagenesis of mouse prostaglandin E2 receptor EP3 subtype in insect cells.

@article{Huang1995ExpressionAS,
  title={Expression and site-directed mutagenesis of mouse prostaglandin E2 receptor EP3 subtype in insect cells.},
  author={Christine S. Huang and Hsin H Tai},
  journal={The Biochemical journal},
  year={1995},
  volume={307 ( Pt 2)},
  pages={493-8}
}
A cDNA encoding for mouse prostaglandin E2 (PGE2) receptor EP3 subtype was cloned from a mouse kidney cDNA library by PCR using terminal primers derived from the known sequence of mouse lung EP3 receptor cDNA. The cloned cDNA was confirmed by sequencing and was expressed in Trichoplusia ni (MG1) insect cells using a baculovirus expression system. A specific protein of 60 kDa was detected by immunoblot with antibodies generated against a unique decapeptide sequence present in the second… CONTINUE READING

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