Expression and secretion of bovine beta-lactoglobulin in Saccharomyces cerevisiae.

  title={Expression and secretion of bovine beta-lactoglobulin in Saccharomyces cerevisiae.},
  author={Mamoru Teruji Totsuka and Yoshio Katakura and Makoto Shimizu and Izumi Kumagai and Kazunobu Miura and Shuichi Kaminogawa},
  journal={Agricultural and biological chemistry},
  volume={54 12},
A bovine beta-lactoglobulin (beta-LG) was expressed in Saccharomyces cerevisiae carrying bovine pre-beta-LG cDNA and secreted into its growth medium. The expression plasmid was constructed by inserting the whole coding region of the cDNA encoding pre-beta-LG between the promoter and terminator of the yeast glyceraldehyde 3-phosphate dehydrogenase gene of pYG100, a yeast expression vector. In the supernatant of the yeast growth medium, beta-LG with a native conformation was detected by sandwich… CONTINUE READING

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