Expression and secretion of an Arthrobacter dextranase in the oral bacterium Streptococcus gordonii.

@article{Kubo1993ExpressionAS,
  title={Expression and secretion of an Arthrobacter dextranase in the oral bacterium Streptococcus gordonii.},
  author={Shinji Kubo and Hisashi Kubota and Yasuo Ohnishi and Takashi Morita and Tokuzo Matsuya and Aizo Matsushiro},
  journal={Infection and immunity},
  year={1993},
  volume={61 10},
  pages={4375-81}
}
We have constructed a plasmid to express and secrete dextranase in the oral bacterium Streptococcus gordonii. The dextranase gene from Arthrobacter sp. strain CB-8 was linked to a promoter and a DNA sequence encoding the signal peptide of Streptococcus downei glucosyltransferase I (gtfI) followed by the Escherichia coli rrnBt1t2 terminator and inserted in the shuttle vector pVA838. S. gordonii transformed with this plasmid (pMNK-4) expressed and secreted mature Arthrobacter dextranase. The… CONTINUE READING
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Effects of dextranases on cell adherence, glucan-film formation and glucan synthesis by Streptococcus mutans glucosyltransferase

  • T. Kop, M. Inoue
  • Arch. Oral Biol
  • 1979
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