Expression and refolding of truncated recombinant major outer membrane protein antigen (r56) of Orientia tsutsugamushi and its use in enzyme-linked immunosorbent assays.

@article{Ching1998ExpressionAR,
  title={Expression and refolding of truncated recombinant major outer membrane protein antigen (r56) of Orientia tsutsugamushi and its use in enzyme-linked immunosorbent assays.},
  author={Wei Mei Ching and Hongxia Wang and Chirapa Eamsila and Daryl J Kelly and Gregory A Dasch},
  journal={Clinical and diagnostic laboratory immunology},
  year={1998},
  volume={5 4},
  pages={519-26}
}
The variable 56-kDa major outer membrane protein of Orientia tsutsugamushi is the immunodominant antigen in human scrub typhus infections. The gene encoding this protein from Karp strain was cloned into the expression vector pET11a. The recombinant protein (r56) was expressed as a truncated nonfusion protein (amino acids 80 to 456 of the open reading frame) which formed an inclusion body when expressed in Escherichia coli BL21. Refolded r56 was purified and compared to purified whole-cell… CONTINUE READING
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