Expression and purification of the synthetic preS1 gene of Hepatitis B Virus with preferred Escherichia coli codon preference.

@article{Qian2006ExpressionAP,
  title={Expression and purification of the synthetic preS1 gene of Hepatitis B Virus with preferred Escherichia coli codon preference.},
  author={Bingjun Qian and Huifeng Shen and Jingjing Xiong and Li Chen and Lu Zhang and Junwei Jia and Yuan Wang and Zhuchuan Zhang and Zheng Yuan and Kaiming Cao and Dabing Zhang},
  journal={Protein expression and purification},
  year={2006},
  volume={48 1},
  pages={
          74-80
        }
}
To produce high levels of hepatitis B virus (HBV) preS1 protein at low cost, a DNA fragment encoding the preS1 region, residues 1-119, of HBV adr subtype was synthesized by overlapping-PCR according to Escherichia coli (E. coli) B preferred codon usage. The synthetic preS1 gene (spreS1) was cloned into the bacterial expression vector pET-30a and transferred into the expression strain E. coli BL21(DE3). Recombinant preS1 protein with an N-terminal His6 tag was expressed at high levels in soluble… CONTINUE READING