Expression and purification of the seven nonstructural proteins of the flavivirus Kunjin in the E. coli and the baculovirus expression systems.

@article{Khromykh1996ExpressionAP,
  title={Expression and purification of the seven nonstructural proteins of the flavivirus Kunjin in the E. coli and the baculovirus expression systems.},
  author={Alexander A Khromykh and Tracey J. Harvey and Mahin Abedinia and Edwin G Westaway},
  journal={Journal of virological methods},
  year={1996},
  volume={61 1-2},
  pages={47-58}
}
All seven nonstructural (ns) proteins of the flavivirus Kunjin (KUN) ranging from NS1 to NS5 were expressed either alone or as fusion proteins with Glutathione-S-transferase (GST). High level expression of recombinant proteins was achieved in Spodoptera frugiperda (Sf9) cells using the baculovirus expression system in contrast to the low level of expression in E. coli. The order of the level of expression of the recombinant fusion proteins per 4 x 10(7) Sf9 cells was: GST-NS5 (yields… CONTINUE READING