Expression and purification of the antimicrobial peptide GSL1 in bacteria for raising antibodies

@inproceedings{Meiyalaghan2014ExpressionAP,
  title={Expression and purification of the antimicrobial peptide GSL1 in bacteria for raising antibodies},
  author={Sathiyamoorthy Meiyalaghan and J. M. Latimer and Andrew V. Kralicek and Martin L Shaw and John G. Lewis and Anthony J. Conner and Philippa J. Barrell},
  booktitle={BMC Research Notes},
  year={2014}
}
The Gibberellin Stimulated-Like (GSL) or Snakin peptides from higher plants are cysteine-rich, with broad spectrum activity against a range of bacterial and fungal pathogens. To detect GSL peptides in applications such as western blot analysis and enzyme-linked immunosorbent assays (ELISA), specific antibodies that recognise GSL peptides are required. However, the intrinsic antimicrobial activity of these peptides is likely to prevent their expression alone in bacterial or yeast expression… CONTINUE READING
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