Expression and purification of the BmK Mm2 neurotoxin from the scorpion Buthus martensii Karsch and its biological activity test.

@article{Fu2004ExpressionAP,
  title={Expression and purification of the BmK Mm2 neurotoxin from the scorpion Buthus martensii Karsch and its biological activity test.},
  author={Yuejun Fu and Bao-feng Chai and Wei Wang and Hui Zhi and Li-tian Yin and Ai-hua Liang},
  journal={Protein expression and purification},
  year={2004},
  volume={38 1},
  pages={
          45-50
        }
}
The gene encoding neurotoxin (BmK Mm2) from the scorpion Buthus martensii Karsch was expressed in Escherichia coli BL21 (DE3) at a level of 1.6 mg/L using expression plasmid pExSecI system. SDS-PAGE analysis of the cell lysate confirmed that gene BmK Mm2 was expressed in soluble form and the expressed production was secreted into Luria-Bertani (LB) culture medium from Escherichia coli. According to the characters of pExSecI expression system, the IgG binding domain-ZZ of Protein A is fused to… CONTINUE READING
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