Expression and purification of recombinant nattokinase in Spodoptera frugiperda cells

  title={Expression and purification of recombinant nattokinase in Spodoptera frugiperda cells},
  author={Xiaoxiang Li and Xiaoli Wang and Shaoling Xiong and Jing Zhang and Litao Cai and Yanyan Yang},
  journal={Biotechnology Letters},
A recombinant baculovirus, rv-egfp-NK, containing a reporter gene encoding the enhanced green fluorescent protein (EGFP), was used to express nattokinase (NK), a fibrinolytic enzyme, in Spodoptera frugiperda (SF-9) cells. The recombinant protein also included a histidine tag for purification using Ni2+ resins. The recombinant NK, ∼30 kDa, retained fibrinolytic activity (60 U/ml). The integration of the EGFP expression cassette in the Bac-to-Bac system is thus an effective method for the… 
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