Expression and purification of recombinant human alpha-defensins in Escherichia coli.

@article{Pazgier2006ExpressionAP,
  title={Expression and purification of recombinant human alpha-defensins in Escherichia coli.},
  author={Marzena Pazgier and Jacek T Lubkowski},
  journal={Protein expression and purification},
  year={2006},
  volume={49 1},
  pages={1-8}
}
Different strategies have been developed to produce small antimicrobial peptides (AMPs) using recombinant techniques. Up to now, all efforts to obtain larger quantities of active recombinant human alpha-defensins have been only moderately successful. Here we report an effective method of biosynthesis of human alpha-defensins (hNP-1 to hNP-3 and hD-5 and hD-6) in the Escherichia coli. All the peptides, expressed as insoluble fusions with the peptide encoded by a portion of E. coli tryptophan… CONTINUE READING

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