Expression and purification of recombinant human zona pellucida proteins.

@article{Harris1999ExpressionAP,
  title={Expression and purification of recombinant human zona pellucida proteins.},
  author={Jeffrey D Harris and C A Seid and Greg K Fontenot and H. F. Liu},
  journal={Protein expression and purification},
  year={1999},
  volume={16 2},
  pages={298-307}
}
Recombinant human zona pellucida (rhZP) proteins (minus the N-terminal leader and the C-terminal transmembrane-like domain) were expressed in four different expression systems: bacteria, yeast, insect cells, and Chinese Hamster Ovary (CHO) cells. The recombinant proteins in each system were engineered with a C-terminal six histidine (His6) segment that was… CONTINUE READING