Expression and purification of a soluble tissue factor fusion protein with an epitope for an unusual calcium-dependent antibody.

@article{Rezaie1992ExpressionAP,
  title={Expression and purification of a soluble tissue factor fusion protein with an epitope for an unusual calcium-dependent antibody.},
  author={Alireza R Rezaie and Maria Maddalena Di Fiore and Pierre F. Neuenschwander and Charles T. Esmon and James H Morrissey},
  journal={Protein expression and purification},
  year={1992},
  volume={3 6},
  pages={453-60}
}
The use of bacterial signal peptides to target recombinant mammalian proteins to the periplasmic space of Escherichia coli (to promote proper disulfide bond formation) has met with variable success. We report the design and use of a bacterial expression vector to direct recombinant fusion proteins to the periplasmic space of E. coli: it contains the signal peptide from the pelB gene of Erwinia carotovora linked to a small peptide epitope for an unusual calcium-dependent antibody (HPC4). HPC4… CONTINUE READING

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