Expression and purification of a small heat shock protein from the plant pathogen Xylella fastidiosa.

@article{Azzoni2004ExpressionAP,
  title={Expression and purification of a small heat shock protein from the plant pathogen Xylella fastidiosa.},
  author={Adriano Rodrigues Azzoni and Susely Ferraz Siqueira Tada and Luciana Kauer Rosselli and D{\'e}bora Pires Paula and Cleide Ferreira Catani and Ad{\~a}o Aparecido Sabino and Jo{\~a}o Alexandre Ribeiro Gonçalvez Barbosa and Beatriz Gomes Guimar{\~a}es and Marcos Nogueira Eberlin and Francisco Javier Enr{\'i}quez Medrano and Anete P Souza},
  journal={Protein expression and purification},
  year={2004},
  volume={33 2},
  pages={297-303}
}
The small heat shock proteins (smHSPs) belong to a family of proteins that function as molecular chaperones by preventing protein aggregation and are also known to contain a conserved region termed alpha-crystallin domain. Here, we report the expression, purification, and partial characterization of a novel smHSP (HSP17.9) from the phytopathogen Xylella fastidiosa, causal agent of the citrus variegated chlorosis (CVC). The gene was cloned into a pET32-Xa/LIC vector to over-express the protein… CONTINUE READING