Expression and purification of a recombinant enantioselective amidase.

Abstract

Microbacterium sp. AJ115 metabolises a wide range of nitriles using the two-step nitrile hydratase/amidase pathway. In this study, the amidase gene of Microbacterium sp. AJ115 has been inserted into the pCal-n-EK expression vector and expressed in Escherichia coli BL21(DE3)pLysS. The expressed protein is active in E. coli and expression of the amidase gene… (More)

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@article{Doran2005ExpressionAP, title={Expression and purification of a recombinant enantioselective amidase.}, author={John Paul Doran and Patrick Duggan and Michael Masterson and Peter D. Turner and Catherine M O'Reilly}, journal={Protein expression and purification}, year={2005}, volume={40 1}, pages={190-6} }