Expression and purification of a putative tumor suppressor gene PP5715 in E. coli with growth inhibition of hepatocellular carcinoma cells.

Abstract

PP5715 is a putative tumor suppressor gene that encodes a protein of 199 amino acids. Expression of PP5715 in E. coli was mainly as inclusion bodies. The recombinant protein was purified by a NTA-Ni2+ affinity column, refolded by dialysis, and shown to suppress growth of two human hepatocellular carcinoma cell lines using the MTT assay or the clonogenic… (More)

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