Expression and molecular characterization of alternative transcripts of the ARHGEF5/TIM oncogene specific for human breast cancer.

@article{Debily2004ExpressionAM,
  title={Expression and molecular characterization of alternative transcripts of the ARHGEF5/TIM oncogene specific for human breast cancer.},
  author={Marie-Anne Debily and Alessandra Camarca and Marina Ciullo and Claudine Mayer and Sandrine El Marhomy and Ismaila Ba and Abdelali Jalil and Anna Maria Anzisi and John J Guardiola and Dominique Piatier‐Tonneau},
  journal={Human molecular genetics},
  year={2004},
  volume={13 3},
  pages={
          323-34
        }
}
The ARHGEF5/TIM oncogene belongs to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho GTPases. It is well established that Rho-GEFs play an important role in tumorigenesis and metastasis through the activation of their substrates, the Rho GTPases. Little is known about ARHGEF5/TIM oncogene expression and cellular functions. Because of its localization close to the common fragile site FRA7I, which has been shown to be responsible for an inverted duplication of the 7q34-q35… Expand
Targeting rho guanine nucleotide exchange factor ARHGEF5/TIM with auto-inhibitory peptides in human breast cancer
TLDR
Structural analysis revealed that the charge neutralization and electrostatic interaction confer additional stability for these two peptide complexes with DH domain, which are found as good binders of DH domain with dissociation constants Kd of 0.35 and 2 µM, respectively. Expand
Expression of Rho Guanine Nucleotide Exchange Factor 39 (ARHGEF39) and Its Prognostic Significance in Hepatocellular Carcinoma
  • Jian Gao, W. Jia
  • Medicine
  • Medical science monitor : international medical journal of experimental and clinical research
  • 2019
TLDR
ARHGEF39 might act as an oncogene in the progression of HCC and might serve as a promising potential prognostic indicator and therapeutic target for HCC. Expand
Rho Guanine Nucleotide Exchange Factor 5 Increases Lung Cancer Cell Tumorigenesis via MMP-2 and Cyclin D1 Upregulation
TLDR
Interestingly, ARHGEF5 levels were significantly associated with tumor grade and pathologic stage, but not age, gender, T stage, or lymph node metastasis status, and was significantly increased in lung adenocarcinoma tissues and cell lines. Expand
TIM, a Dbl-related protein, regulates cell shape and cytoskeletal organization in a Rho-dependent manner.
The Dbl-like guanine nucleotide exchange factors (GEFs) have been implicated in direct activation of the Rho family of small GTPases. We previously isolated transforming immortalized mammary (TIM) asExpand
Emerging Roles of Ephexins in Physiology and Disease
TLDR
The known and proposed functions of Ephexins in physiological and pathological contexts, as well as their regulatory mechanisms are discussed. Expand
MPZL1 forms a signalling complex with GRB2 adaptor and PTPN11 phosphatase in HER2-positive breast cancer cells
TLDR
The data show that the PTPN11 tyrosine phosphatase acts as a scaffold to bridge the association between GRB2 and MPZL1 in a phosphotyrosine-dependent manner, and support the importance of this new signalling complex in the control of cell adhesion of HER2+ breast cancer cells, a key feature of the metastatic process. Expand
Co-expression of Rho guanine nucleotide exchange factor 5 and Src associates with poor prognosis of patients with resected non-small cell lung cancer.
TLDR
ARHGEF5/Src can be considered as a prognostic biomarker and a therapeutic target for patients with resected NSCLC and co-immunoprecipitation revealed that there was a physical interaction between Src and ARHGEf5 in lung cancer cells. Expand
Identification of alternative splicing markers for breast cancer.
TLDR
A new approach for the identification of breast cancer markers that does not measure gene expression but instead uses the ratio of alternatively spliced mRNAs as its indicator is presented, providing a simple alternative for the classification of normal and cancerous breast tumor tissues and underscore the putative role of alternative splicing in the biology of cancer. Expand
The auto-inhibitory state of Rho guanine nucleotide exchange factor ARHGEF5/TIM can be relieved by targeting its SH3 domain with rationally designed peptide aptamers.
TLDR
Peptide binding test and guanine nucleotide exchange analysis solidified that these designed peptides can both bind to the SH3 domain potently and activate TIM-catalyzed RhoA exchange reaction effectively, and a positive correlation between the peptide affinity and induced exchange activity was observed. Expand
Auto-inhibition of the Dbl Family Protein Tim by an N-terminal Helical Motif*
TLDR
This work shows that the Dbl-family protein, Tim, is auto-inhibited by a short, helical motif immediately N-terminal to its DH domain, which directly occludes the catalytic surface of the DH domain to prevent GTPase activation. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 50 REFERENCES
Rac1 in human breast cancer: overexpression, mutation analysis, and characterization of a new isoform, Rac1b
TLDR
Immunohistochemical staining of Rac1 showed weak Rac1 expression in benign breast disease but high expression level in ductal carcinoma-in-situ, primary breast cancer, and lymph node metastases, suggesting activation of Rac 1, in patients with aggressive breast cancer. Expand
Expression cDNA cloning of a novel oncogene with sequence similarity to regulators of small GTP-binding proteins.
TLDR
A cDNA expression library from a human mammary epithelial cell line for detection of novel oncogenes by focus formation assay in NIH3T3 cells was generated and the transforming gene, designated TIM, encoded a predicted protein species of 60 kDa containing a Dbl-Homology (DH) motif. Expand
Rho GTPases are over‐expressed in human tumors
TLDR
Overall, increase in the amount of Rho GTPases, in particular RhoA, appears to be a frequent event in different types of human tumors, which supports the view that RhoGTPases are involved in human carcinogenesis. Expand
Intragenic amplification and formation of extrachromosomal small circular DNA molecules from the PIP gene on chromosome 7 in primary breast carcinomas
TLDR
It is reported that part of the 3′ end, including exon 3, intron C, two‐thirds of exon 4 and a small portion of intron B, is amplified and involved in the formation of extrachromosomal spcDNA molecules in 3/14 breast cancers analyzed. Expand
Overexpression of betaPix-a in human breast cancer tissues.
TLDR
BetaPix-a expression was found to be higher in human breast cancer tissues than in normal breast tissues, which implies a role for betaPIX-a in human Breast tumorigenesis, and it is suggested that betaPx-a may be a useful marker of malignant disease in the breast. Expand
Overexpression of βPix-a in human breast cancer tissues
TLDR
βPix-a expression was found to be higher in human breast cancer tissues than in normal breast tissues, which implies a role for βPIX-a in human Breast tumorigenesis, and it is suggested that βPx-a may be a useful marker of malignant disease in the breast. Expand
Tiam1 mutations in human renal‐cell carcinomas
TLDR
It is shown that for 4 of 5 human renal‐cell carcinoma (RCC) cell lines the expression levels of Tiam1 tended to be inversely correlated with in vitro invasiveness, whereas no obvious correlation could be found between the expression Levels of Rac1 and invasion. Expand
Initiation of the breakage-fusion-bridge mechanism through common fragile site activation in human breast cancer cells: the model of PIP gene duplication from a break at FRA7I.
TLDR
It is shown that the region containing the PIP gene is duplicated in the breast carcinoma cell line T47D, providing the first evidence that this amplification mechanism can be initiated in vivo by fragile site activation. Expand
The potential role for prolactin-inducible protein (PIP) as a marker of human breast cancer micrometastasis
TLDR
It is concluded that PIP mRNA is frequently expressed in both primary human breast tumours and nodal metastases, and the presence of PIP expression in skin creates a potential source of contamination in venepuncture samples that should be considered in its application as a marker for breast tumour micrometastases. Expand
Expression of the gene encoding a prolactin-inducible protein by human breast cancers in vivo: correlation with steroid receptor status.
TLDR
Three lines of evidence are presented that the gene encoding PIP is also expressed by some human breast cancers in vivo: detection of PIP immunoreactivity in the serum of some breast cancer patients; immunohistochemical detection ofPIP in breast cancer sections; and the presence of Pip mRNA, detected by complementary DNA hybridization in human breast biopsy samples. Expand
...
1
2
3
4
5
...