Expression and in vitro activation of Manduca sexta prophenoloxidase-activating proteinase-2 precursor (proPAP-2) from baculovirus-infected insect cells.

@article{Ji2003ExpressionAI,
  title={Expression and in vitro activation of Manduca sexta prophenoloxidase-activating proteinase-2 precursor (proPAP-2) from baculovirus-infected insect cells.},
  author={Chuanyi Ji and Yang Wang and Jeremy Brett Ross and Haobo Jiang},
  journal={Protein expression and purification},
  year={2003},
  volume={29 2},
  pages={
          235-43
        }
}
Prophenoloxidase activation is a component of the immune system in insects and crustaceans. We recently purified and cloned a new prophenoloxidase-activating proteinase (PAP-2) from hemolymph of the tobacco hornworm Manduca sexta [J. Biol. Chem. 278, 3552-3561]. As the terminal component of a putative serine proteinase cascade, this enzyme activates prophenoloxidase (proPO) via limited proteolysis. To purify and study the activating proteinase for PAP-2 from this insect, we expressed the… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 10 CITATIONS

Identification of plasma proteinase complexes with serpin-3 in Manduca sexta.

  • Insect biochemistry and molecular biology
  • 2012
VIEW 1 EXCERPT
CITES METHODS
HIGHLY INFLUENCED

A positive feedback mechanism in the Manduca sexta prophenoloxidase activation system.

  • Insect biochemistry and molecular biology
  • 2008
VIEW 2 EXCERPTS
CITES METHODS