Expression and hydroxyurea-triggered induction of EGFP upon CRISPR/Cas9-mediated integration into the γ-globin gene of K562 cells

@article{Jafari2019ExpressionAH,
  title={Expression and hydroxyurea-triggered induction of EGFP upon CRISPR/Cas9-mediated integration into the γ-globin gene of K562 cells},
  author={Hossein Jafari and Sara Hesami and Mojtaba Safi and Fatemeh Ghasemi and Mehdi Banan},
  journal={Biotechnology Letters},
  year={2019},
  volume={41},
  pages={691-700}
}
ObjectiveTo knock-in an EGFP cassette into the γ-globin genes of K562 cells via CRISPR/Cas9, and to assess expression and hydroxyurea (HU)-mediated induction of the targeted EGFP transgene.ResultsThe EGFP cassettes were specifically knocked into the Gγ gene. EGFP expression was detected in the targeted cell population and isolated clones. Furthermore, EGFP transcript and fluorescence levels were significantly induced following HU-treatment.ConclusionThis system is readily utilizable for genome… CONTINUE READING

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