Expression and functional characterisation of the clpC gene of Mycobacterium leprae: ClpC protein elicits human antibody response.

@article{Misra1996ExpressionAF,
  title={Expression and functional characterisation of the clpC gene of Mycobacterium leprae: ClpC protein elicits human antibody response.},
  author={N. Misra and Saman Habib and Akash Ranjan and Seyed E. Hasnain and I. V. Ambily Nath},
  journal={Gene},
  year={1996},
  volume={172 1},
  pages={99-104}
}
This paper reports the expression of a previously described gene [Nath and Laal, Nucleic Acids Res. 18 (1990) 4935], currently identified as the clpC gene of Mycobacterium leprae, using an in vitro rabbit reticulocyte lysate-coupled transcription/translation system. The produced protein moved as a 95-kDa band on SDS-PAGE. An additional band of 79 kDa was seen which may have resulted from a GTG codon downstream to the initiating ATG in the clpC sequence. A threefold increase in synthesis of the… CONTINUE READING