Expression and functional characterisation of a human chimeric nicotinic receptor with alpha6beta4 properties.

@article{Evans2003ExpressionAF,
  title={Expression and functional characterisation of a human chimeric nicotinic receptor with alpha6beta4 properties.},
  author={Non M Evans and Suchira Bose and Giovanni Benedetti and R. T. de Zwart and Kathy H Pearson and Gordon I McPhie and Peter J. Craig and Jason P Benton and Stephen G Volsen and Emanuele Sher and Lisa M. Broad},
  journal={European journal of pharmacology},
  year={2003},
  volume={466 1-2},
  pages={31-9}
}
Despite being cloned several years ago, the expression of functional nicotinic acetylcholine receptors containing the human alpha6 subunit in recombinant mammalian cell lines has yet to be demonstrated. The resulting lack of selective ligands has hindered the evaluation of the role of alpha6-containing nicotinic receptors. We report that functional channels were recorded following co-transfection of human embryonic kidney (HEK-293) cells with a chimeric alpha6/alpha4 subunit and the beta4… CONTINUE READING