Expression and functional analysis of the anaplastic lymphoma kinase (ALK) gene in tumor cell lines

@article{Dirks2002ExpressionAF,
  title={Expression and functional analysis of the anaplastic lymphoma kinase (ALK) gene in tumor cell lines},
  author={Willy G. Dirks and Silke F{\"a}hnrich and Yvonne Lis and Elisabeth Becker and Roderick A. F. Macleod and Hans G Drexler},
  journal={International Journal of Cancer},
  year={2002},
  volume={100}
}
The initial identification of the ALK gene, expressed as C‐terminal part of the transforming fusion protein NPM‐ALK in the t(2;5)(p23;q35) lymphoma‐associated chromosomal translocation, revealed a novel receptor tyrosine kinase (RTK). In order to expand the knowledge on ALK expression in the human system, we examined a panel of human cell lines for ALK expression and found that transcription is completely repressed in cell lines of entodermal origin (0/21). Furthermore, full length receptor… 
Recombinant expression, characterization, and quantification in human cancer cell lines of the Anaplastic Large-Cell Lymphoma-characteristic NPM-ALK fusion protein
TLDR
It is estimated that N PM-ALK fusion protein is expressed at substantial levels in both Karpas 299 and SU-DHL-1 cells and compared NPM-ALK/ β-actin ratios determined by ELISA to those independently determined by two-dimensional electrophoresis and showed that the two methods are in good agreement.
Ablation of oncogenic ALK is a viable therapeutic approach for anaplastic large-cell lymphomas.
TLDR
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Anaplastic lymphoma kinase activity is essential for the proliferation and survival of anaplastic large-cell lymphoma cells.
TLDR
Select fused pyrrolocarbazole-derived small molecules with ALK inhibitory activity were used as pharmacologic tools to evaluate whether functional ALK is essential for the proliferation and survival of ALK+ ALCL cells in culture.
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TLDR
The role of ALK in development and disease is addressed, implications for the future are discussed and many chromosomal rearrangements leading to enhanced ALK activity are described.
Expression of anaplastic lymphoma kinase in non-Hodgkin's lymphomas and other malignant neoplasms. Biological, diagnostic, and clinical implications.
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  • Medicine
    American journal of clinical pathology
  • 2002
TLDR
Several lines of experimental evidence indicate that the ectopically expressed ALK is oncogenic in ALK+ TCL by being constitutively active owing to autophosphorylation and by stimulating several critical signal transduction pathways involving phospholipase C-gamma, AKT, and STAT3.
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TLDR
Reports of ALK expression in a range of carcinoma‐derived cell lines together with its apparent role as a receptor for PTN and MK, both of which have been implicated in tumourigenesis, raise the possibility that ALK‐mediated signalling could play a role in the development and or progression of a number of common solid tumours.
ALK is a Novel Dependence Receptor: Potential Implications in Development and Cancer
TLDR
The biological significance of the ALK receptor in cancer and development is summarized, in perspective with its dependence receptor function, which could have important implications in the therapy of ALK-positive tumors harboring the chimeric or wild type ALK protein.
Development of anaplastic lymphoma kinase (ALK) small‐molecule inhibitors for cancer therapy
TLDR
A succinct summary of normal ALK biology, the confirmed and putative roles of ALK fusions and the full‐length ALK receptor in the development of human cancers, and efforts to target ALK using small‐molecule kinase inhibitors is provided.
Anaplastic lymphoma kinase: role in cancer pathogenesis and small-molecule inhibitor development for therapy
TLDR
Normal ALK biology, the confirmed and putative roles of ALK in the development of human cancers and efforts to target ALK using small-molecule kinase inhibitors are summarized.
Oncogenic protein tyrosine kinases
TLDR
The study of ALK fusion proteins has raised the possibility of new therapeutic treatments for patients with ALK-positive malignancies, given that ALK fusions also occur in the mesenchymal tumor known as inflammatory myofibroblastic tumor (IMT).
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TLDR
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TLDR
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