Expression and function of TNF-family proteins and receptors in human osteoblasts.

@article{Bu2003ExpressionAF,
  title={Expression and function of TNF-family proteins and receptors in human osteoblasts.},
  author={Rongfa Bu and Christopher W Borysenko and Yanan Li and Lihuan Cao and Afsie Sabokbar and Harry C. Blair},
  journal={Bone},
  year={2003},
  volume={33 5},
  pages={760-70}
}
We studied how tumor necrosis-factor (TNF)-family proteins interact with osteoblasts to resolve several controversial points. We measured expression of TNFs, TNF-receptors, and nonsignaling (decoy) TNF receptors in human osteoblasts derived from mesenchymal stem cells and in MG63 human osteosarcoma cells using unamplified mRNA screening, with secondary Western or PCR analysis where indicated, and studied the effects of TNFs on osteoblasts in cell culture. Expression of TNFs and receptors was… CONTINUE READING
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