Expression and fermentation optimization of oxidized polyvinyl alcohol hydrolase in E. coli

@article{Yang2011ExpressionAF,
  title={Expression and fermentation optimization of oxidized polyvinyl alcohol hydrolase in E. coli},
  author={Yu Kyung Yang and Dongxu Zhang and Song Liu and Dongxu Jia and Guocheng C. Du and Jian Chen},
  journal={Journal of Industrial Microbiology & Biotechnology},
  year={2011},
  volume={39},
  pages={99-104}
}
Oxidized polyvinyl alcohol (PVA) hydrolase (OPH) is a key enzyme in the degradation of PVA, suggesting that OPH has a great potential for application in textile desizing processes. In this study, the OPH gene from Sphingopyxis sp. 113P3 was modified, by artificial synthesis, for overexpression in Escherichia coli. The OPH gene, lacking the sequence encoding the original signal peptide, was inserted into pET-20b (+) expression vector, which was then used to transform E. coli BL21 (DE3). OPH… CONTINUE READING
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