Expression and diagnostic value of proteins in Mycobacterium tuberculosis.

  title={Expression and diagnostic value of proteins in Mycobacterium tuberculosis.},
  author={Zhiqiang Zhu and Y H Liu and Hongmei Wang and Jianjia Xiao and Yiming Qiu and Linli Yan and Du Chen and Airan Liu and Xian-biao Yang},
  journal={Genetics and molecular research : GMR},
  volume={13 3},
We constructed a prokaryotic expression vector expressing the Mycobacterium tuberculosis protein TB16.3, as well as 3 other proteins, including TB15.3, CFP-10, and Rv2626C, which were purified and analyzed for their effectiveness as detection antibodies. The TB16.3 genes of M. tuberculosis H37Rv genomic DNA were amplified by polymerase chain reaction, inserted into the expression vector pET-30a, and expressed in Escherichia coli. An enzyme-linked immunosorbent assay was used to detect the 4 M… CONTINUE READING
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