Expression and characterization of the small subunit of human DNA polymerase delta.

@article{Sun1997ExpressionAC,
  title={Expression and characterization of the small subunit of human DNA polymerase delta.},
  author={Yubo Sun and Ya-jun Jiang and Pengnian Zhang and Shan Jian Zhang and Yonggang Zhou and Bao Qin Li and Ngoc L. Toomey and Marietta Ywt Lee},
  journal={The Journal of biological chemistry},
  year={1997},
  volume={272 20},
  pages={
          13013-8
        }
}
DNA polymerase delta is a heterodimer consisting of a catalytic subunit of 125 kDa and a small subunit of 50 kDa (p50). We have overexpressed p50 in Escherichia coli and have characterized the recombinant protein. p50 was readily overexpressed using the pET vector as an insoluble protein. A procedure was developed for its purification and renaturation. Examination of the physicochemical properties of renatured p50 showed that it is a monomeric protein with an apparent molecular weight of 60,000… CONTINUE READING

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