Expression and characterization of the human erythrocyte anion exchanger in a baculovirus/Sf-9 cell system.

Abstract

The human erythrocyte anion-exchange protein (HAE1) has been expressed in insect Sf-9 cells using a recombinant baculovirus. We subcloned the full-length cDNA encoding HAE1 into the baculovirus expression vector pVL1392 and cotransfected Sf-9 cells with the recombinant vector and wild-type AcMNPV DNA to obtain recombinant baculovirus. The expressed protein… (More)

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@article{Dale1996ExpressionAC, title={Expression and characterization of the human erythrocyte anion exchanger in a baculovirus/Sf-9 cell system.}, author={William E. Dale and Jamie A Textor and Robert W. Mercer and Louis Simchowitz}, journal={Protein expression and purification}, year={1996}, volume={7 1}, pages={1-11} }