Expression and characterization of recombinant murine factor VIII.

  title={Expression and characterization of recombinant murine factor VIII.},
  author={Christopher B. Doering and Ernest T Parker and John F. Healey and Heather N. Craddock and Rachel T. Barrow and Pete Lollar},
  journal={Thrombosis and haemostasis},
  volume={88 3},
Hemophilia A is the inherited bleeding disorder that results from mutation of blood coagulation factor VIII (fVIII). Described here is the generation of a regulated expression system producing recombinant murine fVIII. Murine B-domainless fVIII was expressed at a peak level of 4 units/106 cells/24 h in serum-free media. Subsequently, a two-step purification procedure resulted in 5,300-fold enrichment and a 70% yield. Highly purified recombinant murine fVIII had a specific coagulant activity of… CONTINUE READING
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