Expression and characterization of phosphorylated recombinant human beta-casein in Escherichia coli.

@article{Thurmond1997ExpressionAC,
  title={Expression and characterization of phosphorylated recombinant human beta-casein in Escherichia coli.},
  author={J M Thurmond and Robert G Hards and C T Seipelt and Amanda E. Leonard and Lennart Hansson and Mats Str{\"o}mqvist and Mona Bystr{\"o}m and Kerstin Enquist and Bibo Xu and John J. Kopchick and Pradip Mukerji},
  journal={Protein expression and purification},
  year={1997},
  volume={10 2},
  pages={202-8}
}
Specific serine and threonine residues of recombinant human beta-casein produced in Escherichia coli were shown to be phosphorylated in vivo when human casein kinase II was coexpressed in the same plasmid. All of the phosphorylated forms found in the native protein were also detected in the recombinant protein. The phosphorylation of recombinant human beta-casein was confirmed by immunoblots, fast protein liquid chromatography, urea-polyacrylamide gel electrophoresis, SDS-polyacrylamide gel… CONTINUE READING