Expression and characterization of dog cytochrome P450 2A13 and 2A25 in baculovirus-infected insect cells.

@article{Zhou2010ExpressionAC,
  title={Expression and characterization of dog cytochrome P450 2A13 and 2A25 in baculovirus-infected insect cells.},
  author={Diansong Zhou and Alban J. Linnenbach and Ruifeng Liu and Rick A. Luzietti and Jennifer J. Harris and Catherine L. Booth-Genthe and Scott W. Grimm},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={2010},
  volume={38 7},
  pages={
          1015-8
        }
}
Dog CYP2A13 and CYP2A25 were coexpressed with dog NADPH-cytochrome P450 reductase (OR) in baculovirus-infected Sf9 insect cells. CYP2A13 effectively catalyzed 7-ethoxycoumarin (7EC) deethylation and coumarin hydroxylation with apparent K(m) values of 4.8 and 2.1 microM, respectively, similar to those observed using dog liver microsomes (7.5 and 0.75 microM, respectively). CYP2A25 exhibited much lower affinity toward 7EC, with an apparent K(m) value of 150 microM, which indicates that CYP2A13… CONTINUE READING