Expression and characterization of dog CYP2D15 using baculovirus expression system.

@article{Tasaki1998ExpressionAC,
  title={Expression and characterization of dog CYP2D15 using baculovirus expression system.},
  author={T Tasaki and Akiko M. Nakamura and Shigeru Itoh and Kazuyo Ohashi and Yui Yamamoto and Minoru Masuda and Hisayoshi Iwata and Akio Kazusaka and Tetsuya Kamataki and Shoichi Fujita},
  journal={Journal of biochemistry},
  year={1998},
  volume={123 1},
  pages={
          162-8
        }
}
Dog CYP2D15 was expressed in Sf9 cells with a recombinant baculovirus. Infection of Sf9 insect cells with a recombinant dog CYP2D15-virus resulted in the expression of a protein which cross-reacted with a polyclonal antibody against a dog CYP2D15-specific peptide. The difference spectrum of CO-complex of reduced P450 of the infected cell microsomes had a maximal absorbance at 449 nm. The specific content of P450 was calculated to be 0.56 nmol/mg of Sf9 cell microsomal protein. Although the… CONTINUE READING