Expression and characterization of a recombinant murine coronavirus 3C-like proteinase.

@article{Seybert1997ExpressionAC,
  title={Expression and characterization of a recombinant murine coronavirus 3C-like proteinase.},
  author={Anja Seybert and John Ziebuhr and Stuart G. Siddell},
  journal={The Journal of general virology},
  year={1997},
  volume={78 ( Pt 1)},
  pages={71-5}
}
The replication of coronaviruses involves proteolytic processing of the gene 1 translation products, pp1a and pp1ab. One of the key enzymes in this process is predicted to be a virus-encoded 3C-like proteinase. In this report, we describe a bacterial system that has allowed us to express and characterize a recombinant murine coronavirus (MHV-JHM) 3C-like proteinase. The partially purified protein has been shown to exhibit proteolytic activity in trans and mutation analysis has been used to… CONTINUE READING

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