Expression and characterization of a protein encoded by the human c-myc exon 1 in Escherichia coli.

@article{Psallidopoulos1989ExpressionAC,
  title={Expression and characterization of a protein encoded by the human c-myc exon 1 in Escherichia coli.},
  author={Miltiades C. Psallidopoulos and A. P. Seth and G C Dubois and Robert J. Fisher and Takis S. Papas},
  journal={Gene analysis techniques},
  year={1989},
  volume={6 3},
  pages={47-56}
}
Our previously reported data from DNA sequence studies of the c-myc locus show that the human c-myc exon 1 has an open reading frame capable of encoding a protein of 188 amino acid residues. To confirm the presence of the open reading frame, we constructed a recombinant vector (pMCP60) that contains a segment of the lambda cII translational initiation region, a portion of the N-terminus of the v-mos gene, and 639 base pairs of the first exon of the human c-myc gene. pMCP60 expresses a 38… CONTINUE READING