Expression and characterization of a novel β-glucosidase, with transglycosylation and exo-β-1,3-glucanase activities, from Rhizomucor miehei.

A novel β-glucosidase gene, designated RmBglu3B, was cloned from the thermophilic fungus, Rhizomucor miehei CAU432. Its 2196-bp open reading frame encoded 731 amino acids. Its deduced amino-acid sequence showed highest identity (66%) with a glycoside hydrolase family 3 β-glucosidase from R. miehei NRRL5382. RmBglu3B was successfully expressed in Escherichia… (More)