Expression and characterization of Rv0447c product, potentially the methyltransferase involved in tuberculostearic acid biosynthesis in Mycobacterium tuberculosis.

@article{Meena2013ExpressionAC,
  title={Expression and characterization of Rv0447c product, potentially the methyltransferase involved in tuberculostearic acid biosynthesis in Mycobacterium tuberculosis.},
  author={Laxman Singh Meena and Pappachan E. Kolattukudy},
  journal={Biotechnology and applied biochemistry},
  year={2013},
  volume={60 4},
  pages={412-6}
}
In this study, a previously uncharacterized gene (Rv0447c) of Mycobacterium tuberculosis, designated as an unknown fatty-acid methyltransferase (ufaA1), was cloned, expressed in Escherichia coli, and purified. The biochemical characterization of the purified protein (UfaA1) showed it to be a methyltransferase that catalyzes biosynthesis of the… CONTINUE READING