Expression and characterization of Pseudomonas aeruginosa cytochrome c-551 and two site-directed mutants: role of tryptophan 56 in the modulation of redox properties.

Abstract

The gene coding for Pseudomonas aeruginosa cytochrome c-551 was expressed in Pseudomonas putida under aerobic conditions, using two different expression vectors; the more efficient proved to be pNM185, induced by m-toluate. Mature holo-(cytochrome c-551) was produced in high yield by this expression system, and was purified to homogeneity. Comparison of the… (More)

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@article{Cutruzzol1997ExpressionAC, title={Expression and characterization of Pseudomonas aeruginosa cytochrome c-551 and two site-directed mutants: role of tryptophan 56 in the modulation of redox properties.}, author={Francesca Cutruzzol{\`a} and Iacopo Ciabatti and Gian Paolo Rolli and Silvia Falcinelli and Marco Arese and G. Ranghino and Arianna Anselmino and Elisabetta Zennaro and Mara Silvestrini}, journal={The Biochemical journal}, year={1997}, volume={322 ( Pt 1)}, pages={35-42} }