Expression and characterization of PKD, a phorbol ester and diacylglycerol-stimulated serine protein kinase.

@article{Lint1995ExpressionAC,
  title={Expression and characterization of PKD, a phorbol ester and diacylglycerol-stimulated serine protein kinase.},
  author={Johan Van Lint and James Sinnett-Smith and Enrique 4 Rozengurt},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 3},
  pages={1455-61}
}
A novel protein kinase (named PKD) with an NH2-terminal region containing two cysteine-rich motifs has been expressed in COS-7 cells and identified as a receptor for phorbol esters. COS-7 cells transfected with a PKD cDNA construct (pcDNA3-PKD) exhibit a marked (4.8-fold) increase in [3H]phorbol 12,13-dibutyrate binding. An antiserum raised against the COOH-terminal 15 amino acids of PKD specifically recognized a single 110-kDa band in PKD-transfected cells. PKD prepared by elution from… CONTINUE READING

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