Expression and characterization of Escherichia coli derived hepatitis C virus ARFP/F protein

  title={Expression and characterization of Escherichia coli derived hepatitis C virus ARFP/F protein},
  author={F. Baghbani-Arani and F. Roohvandv and Mohamad Reza Aghasadeghi and A. Eidi and S. Amini and F. Motevalli and S. Sadat and A. Memarnejadian and G. Khalili},
  journal={Molecular Biology},
  • F. Baghbani-Arani, F. Roohvandv, +6 authors G. Khalili
  • Published 2012
  • Medicine, Biology
  • Molecular Biology
  • Genome of the hepatitis C virus (HCV) contains a long open reading frame encoding a polyprotein that is cleaved into 10 proteins. Recently, a novel, so called “ARFP/F”, or “core+1,” protein, which is expressed through a ribosomal frame shift within the capsid-coding sequence, has been described. Herein, to produce and characterize a recombinant form of this protein, the DNA sequence corresponding to the ARFP/F protein (amino acid 11–161) was amplified using a frame-shifted forward primer… CONTINUE READING
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