Expression and characterization of Escherichia coli derived hepatitis C virus ARFP/F protein

@article{BaghbaniArani2012ExpressionAC,
  title={Expression and characterization of Escherichia coli derived hepatitis C virus ARFP/F protein},
  author={Fahimeh Baghbani-Arani and F. Roohvandv and Mohammad Reza Aghasadeghi and Akram Eidi and Safieh Amini and Fatemeh Motevalli and Seyed Mehdi Sadat and Arash Memarnejadian and Ghader Khalili},
  journal={Molecular Biology},
  year={2012},
  volume={46},
  pages={226-235}
}
Genome of the hepatitis C virus (HCV) contains a long open reading frame encoding a polyprotein that is cleaved into 10 proteins. Recently, a novel, so called “ARFP/F”, or “core+1,” protein, which is expressed through a ribosomal frame shift within the capsid-coding sequence, has been described. Herein, to produce and characterize a recombinant form of this protein, the DNA sequence corresponding to the ARFP/F protein (amino acid 11–161) was amplified using a frame-shifted forward primer… 
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TLDR
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