Expression and characteristics of the gene encoding azoreductase from Rhodobacter sphaeroides AS1.1737.

@article{Bin2004ExpressionAC,
  title={Expression and characteristics of the gene encoding azoreductase from Rhodobacter sphaeroides AS1.1737.},
  author={Yan Bin and Zhou Jiti and Wang Jing and Du Cuihong and Hou Hongman and Song Zhiyong and Bao Yongming},
  journal={FEMS microbiology letters},
  year={2004},
  volume={236 1},
  pages={129-36}
}
A gene that encodes a protein with azoreductase activity was obtained by PCR amplification from Rhodobacter sphaeroides AS1.1737. The enzyme, with a molecular weight of 18.7 kD, was heterologously expressed in Escherichia coli and its azoreductase activity was characterized. Furthermore, the reduction mechanism of azo dyes catalyzed by the azoreductase was studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo… CONTINUE READING

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