Expression and biochemical characterization of a multifunctional glycosidase from the thermophilic Bacillus licheniformis SR01.

  title={Expression and biochemical characterization of a multifunctional glycosidase from the thermophilic Bacillus licheniformis SR01.},
  author={Yang Wei and Ya Li and Chun Deng and Shihua Wu and Cuiji Huang and Yi Yi},
  journal={The Journal of general and applied microbiology},
  volume={63 5},
  • Yang WeiYa Li Y. Yi
  • Published 23 August 2017
  • Biology
  • The Journal of general and applied microbiology
A gene (gkdA) (741 bp) encoding a putative protein of 247 amino acids was cloned from the Bacillus licheniformis SR01. The protein was expressed in Escherichia coli BL21 with a molecular mass estimated by SDS-PAGE of approximately 28.03 kDa and showed a calculating isoelectric point (pI) of 6.42. Structure analysis and function identification showed that the enzyme was a multifunctional glycosidase. Its specific activity was 0.013 U/μg. The recombinant glycosidase showed a maximum activity at… 

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