Expression and biochemical characterization of a novel type I pullulanase from Bacillus megaterium

@article{Yang2016ExpressionAB,
  title={Expression and biochemical characterization of a novel type I pullulanase from Bacillus megaterium},
  author={Shaoqing Yang and Qiao-juan Yan and Qingdan Bao and Jingjing Liu and Zheng-qiang Jiang},
  journal={Biotechnology Letters},
  year={2016},
  volume={39},
  pages={397-405}
}
To identify novel pullulanases from microorganisms and to investigate their biochemical characterizations. A novel pullulanase gene (BmPul) from Bacillus megaterium WW1210 was cloned and heterologously expressed in Escherichia coli. The gene has an ORF of 2814 bp encoding 937 amino acids. The recombinant pullulanase (BmPul) was purified to homogeneity and… CONTINUE READING