Expression and activity of 3β-hydroxysteroid dehydrogenase/Δ5-Δ4-isomerase in different regions of the avian brain

@article{Ukena1999ExpressionAA,
  title={Expression and activity of 3β-hydroxysteroid dehydrogenase/Δ5-Δ4-isomerase in different regions of the avian brain},
  author={Kazuyoshi Ukena and Yoko Honda and Yuto Inai and Chie Kohchi and Kazuyoshi Tsutsui},
  journal={Brain Research},
  year={1999},
  volume={818},
  pages={536-542}
}
Abstract Recently, we have demonstrated, using biochemical and immunochemical methods, that the quail brain possesses the cholesterol side-chain cleavage enzyme (cytochrome P450scc) and produces pregnenolone and its sulfate ester. To clarify progesterone biosynthesis in the avian brain, therefore, we examined the expression of messenger RNA (mRNA) encoding for the enzyme 3β-hydroxysteroid dehydrogenase/Δ5-Δ4-isomerase (3β-HSD) and its enzymatic activity using the quail. RT-PCR analysis together… CONTINUE READING

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