Expression and activation of the vacuolar processing enzyme in Saccharomyces cerevisiae.

@article{Hiraiwa1997ExpressionAA,
  title={Expression and activation of the vacuolar processing enzyme in Saccharomyces cerevisiae.},
  author={Noriko Hiraiwa and Mikio Nishimura and Ikuko Hara-Nishimura},
  journal={The Plant journal : for cell and molecular biology},
  year={1997},
  volume={12 4},
  pages={819-29}
}
Vacuolar processing enzymes (VPEs) are cysteine proteinases responsible for maturation of various vacuolar proteins in plants. A larger precursor to VPE synthesized on rough endoplasmic reticulum is converted to an active enzyme in the vacuoles. In this study, a precursor to castor bean VPE was expressed in a pep4 strain of the yeast Saccharomyces cerevisiae to examine the mechanism of activation of VPE. Two VPE proteins of 59 and 46 kDa were detected in the vacuoles of the transformant. They… CONTINUE READING

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