Expression and Secretion of an Acid-Stable α-Amylase Gene in Bacillus Subtilis by SacB Promoter and Signal Peptide

@article{Heng2005ExpressionAS,
  title={Expression and Secretion of an Acid-Stable α-Amylase Gene in Bacillus Subtilis by SacB Promoter and Signal Peptide},
  author={Cai Heng and Zhongjun Chen and Lianxiang Du and Fuping Lu},
  journal={Biotechnology Letters},
  year={2005},
  volume={27},
  pages={1731-1737}
}
Alpha amylase gene from Bacillus licheniformis was mutated by site-directed mutagenesis to improve its acid stability. The mutant gene was expression in Bacillus subtilis under the control of the promoter of sacB gene which was followed by either the α-amylase leader peptide of Bacillus licheniformis or the signal peptide sequence of sacB gene of Bacillus subtilis. Both peptides efficiently directed the secretion of α-amylase from the recombinant B. subtilis cells. The extracellular α-amylase… CONTINUE READING

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