Expression and Secretion of an Acid-Stable α-Amylase Gene in Bacillus Subtilis by SacB Promoter and Signal Peptide

  title={Expression and Secretion of an Acid-Stable α-Amylase Gene in Bacillus Subtilis by SacB Promoter and Signal Peptide},
  author={Cai Heng and Zhongjun Chen and Lianxiang Du and Fuping Lu},
  journal={Biotechnology Letters},
Alpha amylase gene from Bacillus licheniformis was mutated by site-directed mutagenesis to improve its acid stability. The mutant gene was expression in Bacillus subtilis under the control of the promoter of sacB gene which was followed by either the α-amylase leader peptide of Bacillus licheniformis or the signal peptide sequence of sacB gene of Bacillus subtilis. Both peptides efficiently directed the secretion of α-amylase from the recombinant B. subtilis cells. The extracellular α-amylase… CONTINUE READING

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Publications referenced by this paper.
Showing 1-10 of 23 references

Construction of high level inducible expression - secretion system of Bacillus subtilis

W Xu-chu, L Wilson, Louise Tran
Evol . Nat . Sci . • 2001

Enzymes for industrial applications

W Aehle, O Misset
View 1 Excerpt

Potential use of Bacillus subtilis for secretion and production of foreign proteins

RH Doi, SL Wong, F Kawamura
Trends Biotechnol • 1998
View 3 Excerpts

by overlap extension using the polymerase chain reaction

K Ikawa, H Araki, +8 authors S Ito
View 1 Excerpt

Characterization of an endoxylanase produced by an isolated strain of Bacillus sp

LL Lin, WH Hsu
J . Microbiol . Biotechnol • 1997
View 1 Excerpt

Efficient secretion of the authentic mature human growth hormone by Bacillus subtilis

CS Park, CC Chang, JY Kim
J . Biotechnol • 1997
View 1 Excerpt

Hyperexpression of the gene for a Bacillus alpha - amylase in Bacillus subtilis cells : enzymatic properties and crystallization of the recombinant enzyme . Biosci . Biotech - nol . Biochem

JJ Lee, KS Hahm, KY Lee, ST Lee
View 1 Excerpt

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