Expression and Functional Activity of the Bitter Taste Receptors TAS2R1 and TAS2R38 in Human Keratinocytes

@article{Wlfle2015ExpressionAF,
  title={Expression and Functional Activity of the Bitter Taste Receptors TAS2R1 and TAS2R38 in Human Keratinocytes},
  author={Ute W{\"o}lfle and Floriana A. Elsholz and Astrid Kersten and Birgit Haarhaus and W E M{\"u}ller and C M Schempp},
  journal={Skin Pharmacology and Physiology},
  year={2015},
  volume={28},
  pages={137 - 146}
}
Recent studies have shown that human bitter taste receptors (TAS2Rs) are not only expressed in mucous epithelial cells of the tongue, but also in epithelial cells of the colon, stomach and upper respiratory tract. These cell types come in close contact with external bitter compounds by ingestion or breathing. In the present work we addressed the question whether bitter taste receptors might also be expressed in cornified epithelial cells of the skin. Here, we show for the first time the… Expand
Expression and Functional Activity of the Human Bitter Taste Receptor TAS2R38 in Human Placental Tissues and JEG-3 Cells
TLDR
An unexpected strong TAS2R38 expression in the amniotic epithelium, syncytiotrophoblast and decidua cells of the human placenta points to further new functions and hitherto unknown endogenous ligands of Tas2Rs far beyond bitter tasting. Expand
Personalized expression of bitter ‘taste’ receptors in human skin
TLDR
Gene-specific methods are used to measure mRNA from all known bitter receptor genes in adult human skin from freshly biopsied samples and from samples collected at autopsy from the Genotype-Tissue Expression project to contribute to the understanding of extraoral expression of chemosensory receptors. Expand
Personalized expression of bitter ‘taste’ receptors in human skin
The integumentary (i.e., skin) and gustatory systems both function to protect the human body and are a first point of contact with poisons and pathogens. These systems may share a similar protectiveExpand
Analysis of the expression of human bitter taste receptors in extraoral tissues
TLDR
This report will accelerate studies aimed at analyzing the pathophysiological function of T2Rs in different extraoral tissues by suggesting a specific pattern of TAS2R expression with TAS 2R3, 4, 5, 10, 13, 19, and 50 transcripts expressed at moderate levels and TAS1R14 and T2R20 at high levels in the various tissues analyzed. Expand
Functionally expressed bitter taste receptor TAS2R14 in human epidermal keratinocytes serves as a chemosensory receptor
TLDR
It is concluded that TAS2R14 serves as a chemosensory receptor in epidermal keratinocytes and hypothesize that it enables the cells to recognize potentially harmful chemical substances. Expand
Members of Bitter Taste Receptor Cluster Tas2r143/Tas2r135/Tas2r126 Are Expressed in the Epithelium of Murine Airways and Other Non-gustatory Tissues
TLDR
RNA-seq analysis in EGFP-positive cells isolated from the epithelium of trachea and stomach showed expression of genes related to innate immunity, which further support the concept that bitter taste receptors serve functions outside the gustatory system. Expand
Clinical Role of Extraoral Bitter Taste Receptors
TLDR
TAS2R receptors mediate the bronchodilatory effect in human airway smooth muscle, which may lead to the creation of another medicine group used in asthma or chronic obstructive pulmonary disease, and the discovery that functionally compromised TAS2 R receptors negatively impact glucose homeostasis has produced a new area of diabetes research. Expand
The bitter taste receptor TAS2R14 regulates resveratrol transport across the human blood-cerebrospinal fluid barrier.
TLDR
It is demonstrated that the BCSFB is a gateway for resveratrol entrance into the CNS and that the receptor TAS2R14 regulates its transport by regulating the action of efflux transporters at CP epithelial cells. Expand
Amarogentin Displays Immunomodulatory Effects in Human Mast Cells and Keratinocytes
TLDR
The aim of this study was to assess whether bitter compounds display immunomodulatory effects on these immunocompetent cells in the skin, so that they might be a target in chronic inflammatory diseases such as atopic dermatitis and psoriasis. Expand
The bitter truth about bitter taste receptors: beyond sensing bitter in the oral cavity
TLDR
From appetite regulation to the treatment of asthma, regulation of gastrointestinal motility and control of airway innate immunity, the current review gives an overview of the different proposed functions for TAS2Rs in tissues other than the oral cavity. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 21 REFERENCES
Immunohistochemical Detection of TAS2R38 Protein in Human Taste Cells
TLDR
The present study screened a panel of commercially available antisera raised against human bitter taste receptors by immunocytochemical experiments and was able to demonstrate the presence of TAS2R38 protein in human gustatory papillae. Expand
Oligomerization of TAS2R bitter taste receptors.
TLDR
Results show that receptor oligomerization occurs between TAS2R bitter taste receptors; however, functional consequences of hetero-oligomerization were not obvious. Expand
The molecular receptive ranges of human TAS2R bitter taste receptors.
TLDR
The data suggest that the detection of the numerous bitter chemicals is related to the molecular receptive ranges of hTAS2Rs, and some receptors recognized only few agonists, others displayed moderate or extreme tuning broadness. Expand
A Novel Family of Mammalian Taste Receptors
TLDR
It is demonstrated that T2Rs couple to gustducin in vitro, and respond to bitter tastants in a functional expression assay, implying that they function as gust Ducin-linked receptors. Expand
Extraoral bitter taste receptors as mediators of off‐target drug effects
  • Adam A. Clark, S. Liggett, S. Munger
  • Medicine, Biology
  • FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 2012
TLDR
It is proposed that any drug with a bitter taste could have unintended actions in the body through stimulation of extraoral type 2 taste receptors (T2Rs), a novel hypothesis that could explain many off‐target effects of diverse pharmaceuticals. Expand
Receptors and transduction in taste
TLDR
Screening the mass of genome sequence data that have recently become available has provided a new means to identify key receptors for bitter and sweet taste, and molecular biology has also identified receptors for salty, sour and umami taste. Expand
Urea uptake enhances barrier function and antimicrobial defense in humans by regulating epidermal gene expression
TLDR
Results show that urea is a small-molecule regulator of epidermal permeability barrier function and antimicrobial peptide expression after transporter uptake, followed by gene regulatory activity in normal epidermis, with potential therapeutic applications in diseased skin. Expand
Reduced TRPC Channel Expression in Psoriatic Keratinocytes Is Associated with Impaired Differentiation and Enhanced Proliferation
Psoriasis is a characteristic inflammatory and scaly skin condition with typical histopathological features including increased proliferation and hampered differentiation of keratinocytes. TheExpand
Specific TRPC6 Channel Activation, a Novel Approach to Stimulate Keratinocyte Differentiation*
TLDR
Impaired keratinocyte differentiation and proliferation are key elements in the pathophysiology of several important dermatological diseases, including atopic dermatitis and psoriasis and TRPC6 activation by hyperforin may represent a new innovative therapeutic strategy in skin disorders characterized by altered keratinocytes differentiation. Expand
Taste receptor signaling in the mammalian gut.
TLDR
Current evidence that the chemosensory machinery discovered in specialized neuroepithelial taste receptor cells of the lingual epithelium is operational in enteroendocrine open GI cells that sense the chemical composition of the luminal contents of the gut is reviewed. Expand
...
1
2
3
...